The main target from this research aimed to study the effect of some bio-fertilizers on growth and nutritional status of guava transplants in order to reduce the mineral fertilization application during two successive seasons of 2011 and 2012. The obtained results revealed that all investigated treatments significantly increased all vegetative growth measurements (stem height, stem diameter, number of shoots per plant, number of leaves per plant and leaf area) in both seasons of study.Also, results indicated that leaf photosynthetic pigments content (chlorophyll A, B and carotenoids) were increased as well as leaf mineral contents (N, P, K, Ca, Mg, Fe, Mn and Zn) were improved by the different studied treatments. Therefore, it could be concluded that, all investigated bio – fertilizer improve growth and nutritional status especially soil application of Kotengin at 20 g/ plant + phosphorine at 40 g/ plant + Rhizobacterin at 40 g/ plant +K2SO4 at 20 g/ plant followed by soil application of Kotengin at 20 g/ plant + Rhizobacterin at 40 g/ plant + superphosphate at 40 g/ plant +K2SO4 at 20 g/ plant.