Background: Amiodarone (AD) is a strong anti-arrhythmic drug but has adverse reactions on different organs. Fish oil which is rich in Omega-3 polyunsaturated fatty acids (omega-3 PFAs). Aim of the study: to study the toxicity of Amiodaroneon Albinorat lungs and its withdrawal. It also study the possible protective role of fish oil. Materials and Methods: 32 adult male Albino rats were randomly classified into four equal groups. Group I was kept as control group, group II, administered Amiodaronein a daily dose of 30 mg/kg BW; Group III: Received Amiodarone for 6 weeks; stopped for another6 weeks then the rats were sacrificed. Group IV: Given a daily doses of Amiodarone (30 mg/kg BW) with fish oil 150mg/kg BW. Lungs were prepared for histopathological and Immunohistochemical study. Estimation of oxidative markers ((MDA-SOD- GSH) was done. Results: Light microscopical examination of Group II showed distorted pulmonary architecture. Withdrawal of Amiodarone showed very minimal improvement of lung architecture. Group IV showed more or less normal pulmonary architecture with significant decrease of the amount of collagen fibers deposition in comparison to group II. Immunohistochemical results: TGF immuno-expression was positive in group II & III and negative in group IV. Histochemical results showed marked increase in the mean value of MDA level and marked decrease in SOD and GSH levels in Group II and Group III. Protection with fish oil in Group IV reversed this parameter towards the normal values of the control group. Conclusion: Fish oil ameliorates Amiodarone -induced lung toxicity. [Naglaa A. S. Sarg and Kamal M. Kamal. Protection against lung toxicity induced by Amiodarone in Albino rats by fish oil. J Am Sci 2019;15(12):82-92]. ISSN 1545-1003 (print); ISSN 2375-7264 (online). http://www.jofamericanscience.org. 9. doi:10.7537/marsjas151219.09.