Fibronectin and P-selectin are immunohistochemical markers of skin wounds vitality and age estimation. This study aimed to assess and compare between fibronectin and P-selectin as markers of vitality and dating of antemortem (AM) and postmortem (PM) human skin wounds. This study was carried out on 140 human cases that were classified into 3 main groups; Group I [90 cases received at the Emergency Department, Benha University Hospital, Egypt, were sub-classified according to the wound age: AM1 (12 hours; n =20) and Group III [10 normal control skin samples]. Immunohistochemical (IHC) staining technique was performed for all full thickness skin biopsies. The current study confirmed that P-selectin & fibronectin were good markers of wound vitality & designed a scoring system based on the increased intensity of the IHC staining in relation to wound age. The current study concluded that P-selectin is more reliable and specific than fibronectin as a marker of wound age estimation & vitality.