Staff Members - Benha University
Use of RAPD and ISSR markers for the identification of Egyptian Henbane (Hyoscyamus muticus L.) genotypes.
The fourth Conference of Sustainable Agricultural Development, Fayoum University, Faculty of Agriculture 20-22 October 2008. • 2015
Publication Information
Authors
El-Shawaf, I.I.S.; Bekhit, M. M. M.; Hassan, A. M.; El-Saied, F. M. and Masoud,
I. M.
Keywords
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Journal
The fourth Conference of Sustainable Agricultural Development, Fayoum University, Faculty of Agriculture 20-22 October 2008.
Publisher
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Volume
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Issue
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Pages
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publication.type
International
Paper Link
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Supplementary Materials
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Abstract
Three Egyptian henbane genotypes from three different locations (Wadi
Sudr, El Maghara and Shalateen) were screened using two different molecular
Techniques; RAPD and ISSR. Both of these techniques were used to detect
some molecular markers associated with the genotype identification. RAPD
results, from using seven arbitrary primers, revealed 57 amplified fragments, 30
of them were polymorphic (52.63 %). The results revealed the presence of 21
positive and 9 negative molecular markers for the genotypic identification.
ISSR results with five specific primers revealed 41 amplified fragments, 15 of
them were polymorphic (36.59 %). The results revealed the presence of 13
positive and 2 negative molecular markers for the genotypic identification. The
Similarity indices and dendrogram for the genetic distances of the combination
between the two techniques revealed that the highest similarity was 68.90 %
between the henbane genotypes from El Maghara and Shalateen regions.
Meanwhile, the lowest similarity index was between the henbane genotypes
from El Maghara and Wadi Sudr regions (8.90 %). The dendrogram resulting
from the combination of the two techniques separated the three henbane
genotypes into one cluster (including El Maghara and Shalateen genotypes)
with the Wadi Sudr genotype alone.
Sudr, El Maghara and Shalateen) were screened using two different molecular
Techniques; RAPD and ISSR. Both of these techniques were used to detect
some molecular markers associated with the genotype identification. RAPD
results, from using seven arbitrary primers, revealed 57 amplified fragments, 30
of them were polymorphic (52.63 %). The results revealed the presence of 21
positive and 9 negative molecular markers for the genotypic identification.
ISSR results with five specific primers revealed 41 amplified fragments, 15 of
them were polymorphic (36.59 %). The results revealed the presence of 13
positive and 2 negative molecular markers for the genotypic identification. The
Similarity indices and dendrogram for the genetic distances of the combination
between the two techniques revealed that the highest similarity was 68.90 %
between the henbane genotypes from El Maghara and Shalateen regions.
Meanwhile, the lowest similarity index was between the henbane genotypes
from El Maghara and Wadi Sudr regions (8.90 %). The dendrogram resulting
from the combination of the two techniques separated the three henbane
genotypes into one cluster (including El Maghara and Shalateen genotypes)
with the Wadi Sudr genotype alone.