In general, environmental stresses are a serious problem limiting crop productivity in rainfed agriculture. As the world׳s population continues to expand, it is becoming increasingly important to increase food production by improving crop resistance to environmental stresses, through traditional breeding and biotechnology-based germplasm enhancement. In the present study, Lycopersicon esculentum Le-GST gene encoding glutathione s-transferase enzyme was introduced into wheat plants (Triticum aestivum L.) cv. Giza 164. The presence and expression of this gene on the obtained putative transgenic plants and their progeny were confirmed using molecular analysis on structural and functional levels