Abstract The research has been conducted to evaluate the activity of the botanical extracts from Neotorularia aculeolata against Spodoptera littoralis (Biosd.). Five solvents differ in their polarity benzene; ethyl acetate; chloroform; ethanol and water were used to extract the plant material. The following points were studied: (a) Evaluation the toxicity of different extracts against S. littoralis larvae (b) effect of plant extracts on the development of S. littoralis (c) evaluation of the antifeedant activity of plant extracts against 4th instar larvae of S. littoralis. Results showed that the 10% concentration of N. aculeolata exhibited strong antifeedant activity. On the other hand ethanol and benzene extracts caused the highest mortality percentage and antifeedant activity, respectively. The type and concentration of extracts by water, ethanol, chloroform and ethyl acetate affected on the sex ratio and development of S. littoralis. In general, the biological activity of N. aculeolata extracts was solvent type-and concentration-dependent. Key words: Spodoptera littoralis, Neotorularia aculeolata, Brassicaceae, oviposition deterrence, Antifeedant activity, glucosinolates.