In this study, tomato (Carolina Gold cv.) transplants were treated before planting in pots with garlic (G), black pepper (BP) extracts (at 0.5 & 4.0% conc.), salicylic acid (SA) or riboflavin (R) at 0.1 & 10.0mM conc. using different application methods (immersing roots “IR”, spraying shoots “SS” and IR+SS) then inoculated with the tomato wilt fungus Fusarium oxysporum f. sp. lycopercici (FOL). The activities of polyphenoloxidase (PPO) and peroxidase (POD) enzymes were determined in leaves of treated and untreated (control) tomato plants after two months from inoculation. The increase in POD activity was higher and more pronounced than activity of PPO. Comparing to the control treatment, the increase in PPO activity, in most treatments, was ranged between 4.4% (IR/SS/BP at 4%) to 94.2% (SS/R at 0.1mM) although few treatments slightly decreased it. However, all tested treatments increased activity of POD enzyme. The rate of increase was ranged between 60.7 to 1000.0%.