In this study, seedlings of tomato cultivar Carolina Gold were treated by salicylic acid (SA) and riboflavin (R) using different application methods and inoculated with Fusarium oxysporum f.sp. lycopersici. The obtained results revealed that the wilt disease severity (DS) was significantly affected by inducer treatments and application method/treatment interactions. Concerning interactions, the highest significant reduction in DS was recorded by IR/SA @ 0.1mM (94.1%) followed by IR/SA @ 10.0mM, SS/SA @ 10.0mM and IR+SS/R @ 10.0mM (88.2% reduction), IR/R @ 10.0mM, IR+SS/R @ 0.1mM and IR+SS/SA @ 10.0mM (82.4% reduction), IR/R @ 0.1mM and IR+SS/SA @ 0.1mM (70.6% reduction), respectively comparing with the control treatment. Using IR/SA @ 0.1mM caused the highest increase in plant height (53.4%) whereas some interactions such as , IR/R @ 0.1mM, SS/R @ 10.0mM and IR+SS/SA @ 0.1mM showed no significant effects on plant height when compared with the untreated control. The root length recorded the highest significant increase by using SS/SA @ 10.0mM (67.7%) and SS/R @ 0.1mM (65.6%). The lowest significant increase in RL was induced by IR+SS/R @ 0.1mM (21.5%) whereas, IR/R @ 0.1mM and SS/R @ 10.0mM only showed no significant effect on the RL comparing to the untreated control.