Nine Fusarium isolates identified as A, B, C, D, E, F, G, H, and I were isolated from tomato wilted plants grown under glasshouse conditions at different locations of Almaty, Kazakhstan. All isolates formed colonies, conidia and mycelia with morphological characteristics typical of F. oxysporum. These isolates were used for inoculation seedlings of the Carolina Gold cultivar grown in plastic pots under glasshouse conditions. Wilt symptoms particularly brown vascular discoloration in stem were observed after two months from inoculation. This was the first record about presence of tomato wilt caused by Fusarium oxysporum f. sp. lycopersici in Kazakhstan.