Slaughter -house, a building area with special design and construction which meets certain techniques and hygiene requirements. Aim of the study: To identify the effect of poultry slaughter-house environment on workers health in Kalyobia Governorate. Study Design: A descriptive research design was utilized to conduct the study. Setting: The study was conducted at 12 poultry slaughter-houses in Kalyobia Governorate, 3 mechanical and 9 manual. Sample: It included all workers working at 12 poultry slaughter-houses in Kalyobia Governorate, their number is 182. Tool: Two tools were used for collecting data; the first tool is a structured interviewing questionnaire to assess personal characteristics, knowledge about slaughter-houses diseases, and safety environment. The second tool is an observational checklist to assess workers’ practices regarding safety measures. Result: The main findings revealed that most workers had poor knowledge about diseases. Their knowledge about practices was good. Conclusion: The study concluded that the workers had poor knowledge about diseases and approved lack of knowledge and concerns which reflect a need for education about measures for prevention the diseases, the importance of wearing protective measures and following healthy practices in work place. This study recommended that, the health educational message provided to workers in their work place about diseases transmitted from dealing with bird and using safety measure during work.