ABSTRACT The present study was to evaluate anti-cancer effects of Purslane seed oil (PSO) (200g/kg. diet), 5-Flourourasil (20mg/kg b.w. i.p) and their combinatorial formulation in female mice induced by Ehrlich ascites cells for 21 consecutive days prior. Ten days after intraperitoneal inoculation of tumor EAC cells in mice, PSO was supplemented at (200g/kg. diet) daily for 21 consecutive days. On the 22th day, the mice were sacrificed for the estimation of tumor growth (tumor volume), and biochemical parameters (glucose, insulin, alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), lipid peroxides (TBARS), protein thiols (Pr-SHs), reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), total cholesterol (TC), triglycerides (TG), HDL-C, LDL-C, 17β-estradiol and progesterone). The results of this study also showed that administration of Purslane seed oil (PSO), 5-Flourourasil both individually and in combination for 21 days to the carcinoma induced mice demonstrated a significant (P