This study was performed to investigate the effected of experimental work of intestinal surgery for removal caecum of rabbits on alanine amiotransferase (ALT), aspartate aminotransferase(AST), gamma glutamyletransferase (GGT), alkaline phosphatase (ALP), acid phosphotase (ACP), amylase, lipase, catalase (CAT), ceratine phosphokinase (CPK), lactate dehydrogenase (LDH) and cortisol hormone. Two groups of thirteen healthy female New Zland rabbits were used. The first one contained 15 rabbits and kept as control group. The second contain also 15 rabbits subjected to surgery for removal of caecum of rabbits. Blood sample were collected at 3, 6 and 9 days post-operative. The obtained result showed that the increase in serum ALT, AST, GGT, ALP, ACP, amylase, lipase, CAT, CPK, LDH and cortisol hormone were significant increase in experimental group compared with control group. From this result it could be concluded that the biochemical parameter affected with intestinal surgery for removal of rabbit’s caecum.