This study was evaluated the protecting effect of rutin on the glycemic condition, lipid profile, in streptozotocin (STZ)-induced experimental diabetic in rats. With respect to an orally administrated rutin on serum glucose, the result is showing a significant decrease in glucose concentration when compared to diabetic group, While, a significant increase in insulin concentration when compared to diabetic. Moreover, the results revealed that, an administration of streptozotocin (STZ) showing significant increase in triacylglycerol, total cholesterol, LDL-c and VLDL-C concentration when compared with control group, while, showing significant decrease in HDL-C concentration and serum AST and ALT activity when compared to the control group. The data revealed also significant increase in serum LMDA concentration. While, significant decrease in serum calcium concentration when compared to the control group.