Palliative care is currently crucial in nursing care, thus, continuing education in palliative care for practicing nurses is necessary for improving care with a life-limiting illness such as gynecologic cancer. Aim of the study was to investigate the effect of palliative care training package on nurses' performance regarding gynecologic cancer. Sample: A convenient sample of 43 nurses. Research Design: A quasi-experimental (pre-test and post-test) design. Setting: This study was conducted in the Gynecological Oncology Unit of the Maternity Hospital, the department of Gynecology, Outpatient Clinics of the Radiation Oncology and Nuclear Medicine Center at Ain Shams University. Tools of data collection: three tools were utilized including 1) a selfadministered questionnaire that encompassed two parts demographic characteristics and assessment of nurses’ knowledge regarding gynecologic cancer palliative care. 2) Nurses’ attitude towards gynecologic cancer palliative care. 3) observational checklist for nurses’ practice. Results: More than three-quarters of studied nurses had poor knowledge, the majority had a negative attitude and unsatisfactory practice regarding gynecologic cancer palliative care before the training package. However, after one month of the training package, nearly the majority of nurses had good knowledge, a positive attitude, and. satisfactory practice. A statistically positive correlation between total knowledge, attitude, and practice scores regarding palliative care for gynecological cancer before and after training package (P ≤0.01). Conclusion: The training package had a favorable effect on nurses' performance regarding gynecologic cancer palliative care. Recommendation: Continuous in-service training on palliative care for nurses is essential to improve performance in the care of women with gynecological cancer.