Gynaecological examination is essential part of gynaecological care and is the most commonly performed procedure in gynaecological practice. Aim: the present study was aimed to evaluate the effect of educational intervention on maternity nurses’ performance regarding gynaecological examination. Design: Aquasi-experimental design was utilized to fulfill the aim of the study. Settings: The study was conducted at obstetric and gynaecological department and outpatient clinic at Benha university hospital. A sample: Convenient sample of 62 maternity nurses were included in the present study. Data: were collected through two tools: 1) an interviewing questionnaire sheet which includes two parts: a) nurse's personnel characteristics. b) nurse's knowledge regarding gynaecological examination. 2) Observational check list to evaluate the practice of maternity nurse. Results: the result of present study proved that the mean age of maternity nurse were 29.6±7.62 and more than three quarters (77.4%) of them had no previous courses regarding gynaecological examination. Additionally there were highly statistical difference between total knowledge and practical scores of maternity nurses at pre and post intervention as knowledge and practice score at pre intervention were (53.2% - 77.4%) respectively. That improved post intervention (66.1%-88.7) respectively. Conclusion the educational intervention is highly improved maternity nurses' performance (knowledge& practice) regarding gynaecological examination. Recommendations: The present study recommended that educational program regarding gynaecological examination should be provided for all obstetric health care givers.