Staff Members - Benha University
anthelmenthic effect of tamoxifen on experimentally h nana infected mice
egyptian society of parasitology • 2020
Publication Information
Authors
amay el fakahany,mohamed hussien,atef hussien.naglaa ashraf,hemat salah
Keywords
Not Available
Journal
egyptian society of parasitology
Publisher
Not Available
Volume
Not Available
Issue
Not Available
Pages
Not Available
publication.type
International
Paper Link
Not Available
Supplementary Materials
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Abstract
Background: Cystic echinococcosis is a frequent worldwide disease. Surgery is the main policy of
treatment. The benzimidazoles are the drugs of choice as adjuvant therapy besides surgery, but they have
some drawbacks, so there is a pressing need to develop other safe and cheap drugs such as an herbal
extract.
Objectives: To increase the efficacy of Nigella sativa oil (NSO) administered either as monotherapy or
combined with albendazole, and using chitosan nanoparticles as a drug delivery system for both drugs.
Material and Methods: E. granulosus protoscoleces were obtained by aseptic puncture of hydatid
cysts. Mice infected by intraperitoneal inoculation with nearly 1000 protoscoleces per mouse were
subdivided into 7 groups: G2, infected non-treated; G3, treated with albendazole; G4, treated with NSO;
G5, treated with NSO and albendazole; G6, treated with albendazole loaded on chitosan nanoparticles;
G7, treated with NSO loaded on chitosan nanoparticles; G8, treated with albendazole and NSO loaded on
chitosan nanoparticles. G1 included the non-infected, non-treated control mice. Serum was collected for
biochemical and serological assay. NO was measured according to nitric oxide assay colorimetric kit. The
systemic level of TNF-α and IL-5 was determined in the plasma of mice using commercial enzyme-linked
immunosorbent assay kits.
Results: The results revealed that a significant increase of IL5 occurred in all treated groups to reach the
highest value in G8. There was a significant decrease of TNF-α in all treated groups in comparison to G1
and G2 (P
treatment. The benzimidazoles are the drugs of choice as adjuvant therapy besides surgery, but they have
some drawbacks, so there is a pressing need to develop other safe and cheap drugs such as an herbal
extract.
Objectives: To increase the efficacy of Nigella sativa oil (NSO) administered either as monotherapy or
combined with albendazole, and using chitosan nanoparticles as a drug delivery system for both drugs.
Material and Methods: E. granulosus protoscoleces were obtained by aseptic puncture of hydatid
cysts. Mice infected by intraperitoneal inoculation with nearly 1000 protoscoleces per mouse were
subdivided into 7 groups: G2, infected non-treated; G3, treated with albendazole; G4, treated with NSO;
G5, treated with NSO and albendazole; G6, treated with albendazole loaded on chitosan nanoparticles;
G7, treated with NSO loaded on chitosan nanoparticles; G8, treated with albendazole and NSO loaded on
chitosan nanoparticles. G1 included the non-infected, non-treated control mice. Serum was collected for
biochemical and serological assay. NO was measured according to nitric oxide assay colorimetric kit. The
systemic level of TNF-α and IL-5 was determined in the plasma of mice using commercial enzyme-linked
immunosorbent assay kits.
Results: The results revealed that a significant increase of IL5 occurred in all treated groups to reach the
highest value in G8. There was a significant decrease of TNF-α in all treated groups in comparison to G1
and G2 (P