The study was aimed to assess the effect of offering a holistic package of quality nursing care for nurses caring patients undergoing gastrointestinal (GI) endoscopy, to verify patient's safety. Research Design: Quasi experimental design was used to meet the aims of this study. Setting: study was conducted in the Gastroenterology Endoscopic units at Benha University Hospital. Sample: a convenience sample of all nurses (30) who are working in gastroenterology Endoscopic units at Benha University Hospital. Tools: Three tools were utilized. 1st tool: Nurses' Knowledge Questionnaire, it includes two parts: Part 1: demographic data for nurses, Part 2: Nurse's knowledge test: to assess nurse's knowledge about care of patients undergoing upper GI endoscopy, 2nd tool: Nurses' Observational Checklist to assess the nurses' practices for caring of patient undergoing upper GI endoscopy, 3rd tool: The designed holistic package of quality nursing care for nurses caring patients undergoing GI endoscopy. The main results: the study demonstrated that there was highly statistically improvement in knowledge and practice of nurses caring patients undergoing GI endoscopy, also there was positive correlation between nurses' knowledge and practice scores, in addition to there was significant positive correlation between knowledge and practice scores with years of experiences. Conclusion: The present findings concluded that there were very highly statistical significant improvement in all items that related to infection control and patients' safety measures throughout study phases. Recommendations: The holistic package of quality nursing care should be applied & evaluated periodically on endoscopic units. Further researchers; conducting educational training programs about enhancing safety measures, infection control, and patients' right for patient undergoing GI endoscopy