Nephrotoxicity is the main problem associated with gentamicin. So this work aimed to evaluate the effect of Vitamin C on gentamicin -induced nephrotoxicity in Baladi goats (Capra hircus) based on the clinical, haematobiochemical and histopathological changes. To achieve this aim, ten clinical healthy Baladi goats were allotted into two groups, each of five. The first group was gentamicin-induced nephrotoxicity that injected with gentamicin while, the second group was injected with Vitamin C along with gentamycin. Jugular blood samples were collected at 0, 7, and 12 days post gentamycin injection. Dullness, decreased appetite and unable to stand and lie down were the main signs exhibited after gentamicin injection. However, these signs did not appear in Vitamin C treated goats. In addition, there was significant increase in serum urea, creatinine and serum MDA. While hematological examination showed a significant decrease in Hb content and RBCs content and erythrocyte SOD. In the meantime, histopathological examinations revealed severe renal damage. Marked improvement in the haematobiochemical changes were detected in gentamicin Vitamin C treated goats with mild renal damage. Our results suggest the cytoprotective role of Vitamin C on gentamicin-induced nephrotoxicity in goats that could be attributed to its anti-oxidant activity, a result of high importance in clinical application.