Staff Members - Benha University
The association between Folate metabolism, C677T and A1298C Polymorphisms of Methylene tetrahydrofolate reductase Gene and the genetic susceptibility of Preeclampsia among a sample of Jordanian pregnant women
• 2021
Publication Information
Authors
samir S Mahgoub1, Omar A Azzam2
, Abufraijeh SM3
,
Al-Kharabsheh AM4
, Heba M Abd El Kareem5
Keywords
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publication.type
Local
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Supplementary Materials
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Abstract
Background: Preeclampsia (PE) is a pregnancy disorder causes uteroplacental
dysfunction resulting in fetal growth restriction. Aim: This study aimed to
evaluate the association between C677T and A1298C polymorphisms of
methylene tetrahydrofolate reductase (MTHFR) gene and PE susceptibility in
a sample of Jordanian women. Methodology: The analysis of MTHFR C677T
and A1298C polymorphisms was done by Polymerase Chain ReactionRestriction Fragment Length Polymorphism (PCR-RFLP). PCR-RFLP products
were digested with hinfI and MboII enzymes. The digestion products were
electrophoresed on 2% agarose gel, stained and visualized under UV light.
The levels of homocysteine, folic acid, and B12 were assayed using ELISA.
Results: The study revealed a significant difference in plasma levels of
homocysteine in PE patients versus the controls. TT, CT polymorphisms, AC
polymorphism, mutant T, C alleles of MTHFR C677T and A1298C
polymorphisms, respectively, also, combined polymorphisms 677CT/1298CC,
677CT/1298AA and 677TT/1298AA were shown to be associated with
increased PE susceptibility. Conclusion: Our data suggest that MTHFR C677T
and A1298C polymorphisms are most likely to be risk factors for developing
PE among Jordanian pregnant women. Also, the two types of polymorphisms
may synergize to increase the risk of PE.
dysfunction resulting in fetal growth restriction. Aim: This study aimed to
evaluate the association between C677T and A1298C polymorphisms of
methylene tetrahydrofolate reductase (MTHFR) gene and PE susceptibility in
a sample of Jordanian women. Methodology: The analysis of MTHFR C677T
and A1298C polymorphisms was done by Polymerase Chain ReactionRestriction Fragment Length Polymorphism (PCR-RFLP). PCR-RFLP products
were digested with hinfI and MboII enzymes. The digestion products were
electrophoresed on 2% agarose gel, stained and visualized under UV light.
The levels of homocysteine, folic acid, and B12 were assayed using ELISA.
Results: The study revealed a significant difference in plasma levels of
homocysteine in PE patients versus the controls. TT, CT polymorphisms, AC
polymorphism, mutant T, C alleles of MTHFR C677T and A1298C
polymorphisms, respectively, also, combined polymorphisms 677CT/1298CC,
677CT/1298AA and 677TT/1298AA were shown to be associated with
increased PE susceptibility. Conclusion: Our data suggest that MTHFR C677T
and A1298C polymorphisms are most likely to be risk factors for developing
PE among Jordanian pregnant women. Also, the two types of polymorphisms
may synergize to increase the risk of PE.