Staff Members - Benha University
Is there an Association Between-2549 Insertion/Deletion Polymorphisms in the Promotor Region of the Gene Encoding for VEGFA as a Risk Factor and the Idiopathic Recurrent Spontaneous Miscarriage in a Sample of Jordanian Women?
• 2020
Publication Information
Authors
Azzam OA1
*, Mahgoub SS2,3, Farhan SS4
, Alrawashdeh HM5
, Abufraijeh SM6
and Abd El kareemHM7,8
Keywords
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Journal
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Publisher
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Issue
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Pages
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publication.type
Local
Paper Link
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Supplementary Materials
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Abstract
Background: At least 50% of the cases of recurrent spontaneous miscarriage is aetiologically idiopathic. Recently various genetic polymorphisms have been
proposed as susceptibility risk factors for pregnancy loss.
Objective: The aim of the present case control-study is to establish the association between the functional -2549 I/D polymorphisms in the promoter region of the
vascular endothelial growth factor A (VEGFA) gene and idiopathic recurrent spontaneous miscarriage (IRSM) in a sample of Jordanian women.
Subjects and Methods: 328 subjects were recruited, 103 and 98 women with primary and secondary IRSM, respectively, 127 normal women were selected as
a control group. Genomic DNA was isolated from a blood sample withdrawn from each participant, then, -2549 I/D polymorphisms of the VEGFA gene were
genotyped by Polymerase Chain Reaction (PCR).
Results: The obtained results revealed that ID polymorphism and D allele of VEGFA -2549 I/D polymorphisms have the highest frequencies in both primary and
secondary IRSM patients, no significant difference between the three groups regarding polymorphisms and allele frequencies, patients with DD+ID genetic models
have positive association with high risk of IRSM versus II model, and patients with D allele are more liable to have IRSM than those having I allele, no significant
difference in the association of VEGFA -2549 I/D polymorphisms with IRSM in the three genetic models of the primary and secondary IRSM patients.
Conclusion: patients with ID genetic model of -2549 I/D polymorphisms in the VEGFA gene’s promoter region and D allele have a higher risk for IRSM.
proposed as susceptibility risk factors for pregnancy loss.
Objective: The aim of the present case control-study is to establish the association between the functional -2549 I/D polymorphisms in the promoter region of the
vascular endothelial growth factor A (VEGFA) gene and idiopathic recurrent spontaneous miscarriage (IRSM) in a sample of Jordanian women.
Subjects and Methods: 328 subjects were recruited, 103 and 98 women with primary and secondary IRSM, respectively, 127 normal women were selected as
a control group. Genomic DNA was isolated from a blood sample withdrawn from each participant, then, -2549 I/D polymorphisms of the VEGFA gene were
genotyped by Polymerase Chain Reaction (PCR).
Results: The obtained results revealed that ID polymorphism and D allele of VEGFA -2549 I/D polymorphisms have the highest frequencies in both primary and
secondary IRSM patients, no significant difference between the three groups regarding polymorphisms and allele frequencies, patients with DD+ID genetic models
have positive association with high risk of IRSM versus II model, and patients with D allele are more liable to have IRSM than those having I allele, no significant
difference in the association of VEGFA -2549 I/D polymorphisms with IRSM in the three genetic models of the primary and secondary IRSM patients.
Conclusion: patients with ID genetic model of -2549 I/D polymorphisms in the VEGFA gene’s promoter region and D allele have a higher risk for IRSM.