Thirteen antagonistic fungi were isolated from naturally infested soil with nematodes, purified, identified and grown on Gliotoxin fermentation medium. The grown isolates were sterile- filtered to remove fungal biomass and the filtrates were placed into 24- well plates to test their effects on the hatch of Meloidogyne incognita egg-masses Also, effects of these filtrates and sporse of the used thirteen antagonistic fungi were evaluated in greenhouse test on the reproduction of M. incognita and the growth of soybean Giza 35 . Meloidogyne incognita egg- masses hatch ranged from 25.7% when using Arthrobotrys oviformis to 98.3 when using Trichoderma harzianum compared with untreated control. Greenhouse application with fungal filtrates ranged from zero for several filtrates against juvenile of root-knot in soil, number of galls and egg- masses on soybean roots to 4500 juvenils, 437 galls and 288 egg-masses for Gliocladium penicilloides Applications with fungal spores ranged also from zero to 5567 juvenils, 236 galls and 141 egg-masses for the same fungi. The response of shoot and root weights and lengths differs among the treatments.