River Nile (Rossita Branch) receives massive quantities of untreated or inadquite municipal wastewater, industrial and agricultural wastewater through El-Rahawy drain. Consequently, the objective of the present study was to design a system depending on biological treatment followed by Fenton reaction for treatment of El-Rahawy drain wastewater. The physicochemical and bacteriological parameters were estimated for El Rahawy drain wastewater and treated effluent of the drainage wastewater. The samples were collected after 24h and after the fourth day of the treatment. The bioaugmented bacterial consortium that used to enhance the biological treatment was nonpathogenic and eco-friendly, and the inoculated dose was 10 gm/m3/day. The removal efficiency of TDS reached 58.9% and 68.6 % by biological treatment after 24 h and 4 days. While the removal % of TDS reached 75.9% after 24 h of Fenton treatment, and this efficiency removal declined to 66.6% on the fourth day of the treatment. The removal efficiency of COD reached 33.3 and 56.8% after 24 h and 4 days of the biological treatment. Whereas the removal % of COD reached to 80% after 24 h, and this removal % declined to 78.8% after 4 days of Fenton treatment. The physicochemical and bacteriological parameters were declined by the treatment time increase. It was found that, the bioaugmented bacterial strains are able to grow well and produce more biodegeding enzymes after 24 h. Moreover, the biological treatment followed by the Fenton reaction is the most suitable solution for El-Rahawy drain treatment