Continuous Positive Airway Pressure (CPAP) is the most common form of non-invasive respiratory support applied to preterm infants and is a standard of care. Aim of the study was to evaluate the training clinical positioning guidelines on physiological parameters of premature infants undergoing Nasal Continuous Positive Airway Pressure. Design: A quasi - experimental research design. Setting: The study was carried out at Neonatal Intensive Care Units affiliated toBenha specialized hospital of children at Benha city. Sample: .A convenient sample of 50 nurses working at the study setting and a purposive sample of 50 neonates who undergoing CPAP. Tools: Three instruments were utilized to collect data; A structured interviewing questionnaire sheet, an observational assessment checklists. Results: this study revealed that, nursing intervention in the form of body positioning guidelines improved medical outcomes of preterm infants who placed on CPAP on post program phase compared with pre program phase. Conclusion: preterm infants who exposed to the intervention program in the form of body positioning had better mean scores in relation to their medical outcomes post program compared with preprogram intervention. . Recommendation, Program intervention in the form of body positioning should be integrated as a part of routine daily care of infants with nasal continuous positive airway pressure