Objectives: Peri-Operating Nurses are exposed to various hazards in Operating Rooms (ORs). This study aimed to identify occupational hazards in ORs. Methods: This cross-sectional study targeted all nurses staff-working in ORs in Benha University Hospitals, Egypt, from 1stMarch to 31stAugust 2016. Information on demographic data, work situation, occupational hazards in ORs was collected using an interview questionnaire. Results: The study recruited 167female nurses. Their mean age was 32.77±8.42 years. The majority of nurses was acquainted with the concept of OR hazards and reported that they were adversely affected by these hazards. The risk of exposure was high for radiation, blood and nosocomial diseases and significant for exposure to anesthetic drugs, latex allergy and muscle pain due to awkward positions. Conclusion: The complex structure of ORs leads to increased occupational hazards which affect operating room nurses’ health.