The toxic effect of hydroxylated C60 fullerene (fullerenol) in liver of swiss albino mice has been studied using 100 and 500 mg/kg of hydroxylated C60. Polyhydroxylated fullerenes, C60(OH)29, was synthesized in high yield through a high-speed vibration milling method and characterized using FTIR, mass spectroscopy, and thermal gravimetric analysis to estimates the number of hydroxyl groups attached to the fullerene cage. This investigation aimed to evaluate the toxicity of fullerenol in the liver of mice. Mice were divided into 3 groups, the first group served as control group, second and third groups received oral administration doses of 100 and 500 mg/kg of fullerenol, respectively, for 8 consecutive days. The results revealed that dose of 100 mg/kg showed less toxic effects represented by relative healthy liver with ability of regeneration, minor fibrosis, apoptosis geneexpression and no oxidative stress gene-expression were seen. Whereas, dose of 500 mg/kg of fullerenol caused pathological liver changes with heavy presence of apoptosis and oxidative stress gene-expressions.