The aim of the study was to evaluate the single and multiple follicle development dynamics in three different physiological herds (pubertal, sexually matured and post-partum stages) of Chinese crossbred buffaloes (Nili-Ravi × Murrah × China indigenous swamp) and their relationship with Growth, regression, ovulation, initial and maximum size of dominant follicle (DF). 66 animals were ultrasonically examined throughout the estrous cycle. Firstly follicles were detected to be ≥4 mm in single and multiple follicle development at puberty, sexual maturity and post-partum stages. The results revealed that most of animals showed two waves of follicular dynamics during estrus cycle. These animals (n=59) were exposed to further study of follicular dynamics excluding animals showed one and three waves cycles. Postpartum buffaloes presented multiple follicle development has the significantly biggest length of estrus cycle (21.9±2.1) in comparison to sexually matured and Pubertal herds. However, there is detected no significant difference of estrus cycle length between single (20.1±2.8) and multiple (20.3±3.3) follicle development. Moreover, the number of follicle wave cycle is closely related to the estrus cycle length. The overall linear DF growth rate was significantly bigger (P < 0.05) in second wave multiple recruited follicles at post-partum period as compared to that of sexually matured and pubertal period. The biggest Initial and Maximum size found multiple recruited follicles at post-partum stage was 5.9±0.7 mm and 12.2±2.1 mm, respectively. Therefore, selecting the two wave cycle, with multiple recruited follicles will improve buffalo fertility by using MOET, superovulation and FTAI in Crossbred Buffaloes.