Staff Members - Benha University
Significance of stem cell marker Lgr5 expression in colorectal carcinogenesis
Medical Journal of Cairo University • 2013
Publication Information
Authors
Ranih Zakaria Amer, Nashwa Mohamed Emara, Ghada Abd-Elfattah, Heba Mohamed Rashad, Nehal Saad ZaferPathology Department, Benha Faculty of Medicine, Benha University
Keywords
Not Available
Journal
Medical Journal of Cairo University
Publisher
Not Available
Volume
81
Issue
2
Pages
Not Available
publication.type
Local
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
BACKGROUND:
Lgr5 (Leucine rich repeat containing G–protein- coupled receptors) also known as G-protein- coupled receptor (Gpr-49). It belongs to the large, G-protein– coupled, 7-transmembrane (7TM) family of proteins. In order to determine whether Lgr5 is a potential marker of cancer stem cells, we investigated Lgr5 expression in normal colorectal mucosa, adenoma and carcinoma and compared the results with the clinicopathological data and prognosis.
METHODS:
This is a retrospective study carried upon 40 Egyptian patients with different types of colorectal lesions, 6 cases of adenoma and 34 carcinoma (20 cases were cribriform adenocarcinoma, 6 signet ring carcinoma and 8 mucoid carcinoma). Six cases were taken as control from adjacent normal mucosa in patients with intestinal infarction. Lgr5 expression was evaluated by immunohistochemistry in the collected colorectal lesions.
RESULTS:
Lgr5 was detected as cytoplasmic brown granules with different expression patterns in normal colonic mucosa, adenoma and carcinoma. Lgr5 expression was significantly higher in carcinoma than in adenoma (P
Lgr5 (Leucine rich repeat containing G–protein- coupled receptors) also known as G-protein- coupled receptor (Gpr-49). It belongs to the large, G-protein– coupled, 7-transmembrane (7TM) family of proteins. In order to determine whether Lgr5 is a potential marker of cancer stem cells, we investigated Lgr5 expression in normal colorectal mucosa, adenoma and carcinoma and compared the results with the clinicopathological data and prognosis.
METHODS:
This is a retrospective study carried upon 40 Egyptian patients with different types of colorectal lesions, 6 cases of adenoma and 34 carcinoma (20 cases were cribriform adenocarcinoma, 6 signet ring carcinoma and 8 mucoid carcinoma). Six cases were taken as control from adjacent normal mucosa in patients with intestinal infarction. Lgr5 expression was evaluated by immunohistochemistry in the collected colorectal lesions.
RESULTS:
Lgr5 was detected as cytoplasmic brown granules with different expression patterns in normal colonic mucosa, adenoma and carcinoma. Lgr5 expression was significantly higher in carcinoma than in adenoma (P