Staff Members - Benha University
Diagnosis of Toxoplasmosis Using Surface Antigen Grade 1 Detection by ELISA, Nano-Gold ELISA, and PCR in Pregnant Women
• 2023
Publication Information
Authors
Nagwa SM Aly1,2, Hye-Sook Kim2, Yasmin M Marei3, Azza S Elhamshary1, Ibrahim R Bayoumi4,
Rabab E Omar1, Dina A Mohammed1, Shin-Ichi Miyoshi5, Gehan A Rashed1
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publication.type
International
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Abstract
The accurate diagnosis of toxoplasmosis has critical importance in pregnant women. Nanotechnology and molecular
biology are making possible opportunities for accurate and rapid diagnosis of many infectious diseases.
Aim and Methods: The aim of our study was to compare nano-gold ELISA with ELISA and PCR for diagnosis of toxoplasmosis
using Toxoplasma surface antigen grade 1 (SAG1) in pregnant women seeking antenatal care in outpatient clinics.
Results: PCR showed the highest diagnostic values than nano-gold ELISA and ELISA regarding sensitivity (97.3% versus 89.2% and
83.8%); specificity (100% versus 94% and 88%); and diagnostic accuracy (98.9% versus 91.95% and 86.2%), respectively. There is no
statistical difference between PCR and nanogold ELISA results.
Discussion: Nano-gold ELISA had a significant improvement in diagnosis than the traditional ELISA method. Most likely with the
assistance of nanoparticles, more antibodies enter the antigen–antibody complex because of the considerable improvement in the
surface area of nano-gold particles.
Conclusion: Although PCR had higher diagnostic values than nano ELISA, nano ELISA is cheaper and easier than PCR. We
recommend nano-gold ELISA with SAG1 as a promising technique in the diagnosis of toxoplasmosis and survey studies
biology are making possible opportunities for accurate and rapid diagnosis of many infectious diseases.
Aim and Methods: The aim of our study was to compare nano-gold ELISA with ELISA and PCR for diagnosis of toxoplasmosis
using Toxoplasma surface antigen grade 1 (SAG1) in pregnant women seeking antenatal care in outpatient clinics.
Results: PCR showed the highest diagnostic values than nano-gold ELISA and ELISA regarding sensitivity (97.3% versus 89.2% and
83.8%); specificity (100% versus 94% and 88%); and diagnostic accuracy (98.9% versus 91.95% and 86.2%), respectively. There is no
statistical difference between PCR and nanogold ELISA results.
Discussion: Nano-gold ELISA had a significant improvement in diagnosis than the traditional ELISA method. Most likely with the
assistance of nanoparticles, more antibodies enter the antigen–antibody complex because of the considerable improvement in the
surface area of nano-gold particles.
Conclusion: Although PCR had higher diagnostic values than nano ELISA, nano ELISA is cheaper and easier than PCR. We
recommend nano-gold ELISA with SAG1 as a promising technique in the diagnosis of toxoplasmosis and survey studies