Abstract Background: COPD may be associated with other systemic diseases including cardiovascular diseases, diabetes, osteoporosis and peptic ulceration and Helicobacter pylori infection seems to be the main cause of PUD. Aims: The aim of this work is to study the association of H. pylori infection in chronic obstructive pulmonary disease (COPD). Subject and methods: This study was performed on 80 subjects. They were classified into two groups: Group I: 65 COPD patients. Group II: 20 healthy control subjects. COPD was diagnosed according to the Global Initiative for chronic obstructive pulmonary disease admitted at chest department of Benha University Hospital. Results: It shows that seropositivity of anti-H. pylori IgG and anti-CagA IgG was higher in COPD patients than in controls with a highly statistically significant difference (p =0.009 and 0.047 respectively). Also the IgG level of H. pylori positive cases and CagA positive cases was higher in the COPD group than in the control group with a highly statistically significant difference (p= 0.027 and 0.0001 respectively). Conclusion: The present study suggests that patients with COPD have an increased seroprevalence of Hp infection.