The present study was conducted on 31 buffalo-cows aged 3-10 years and diagnosed rectally as they had follicular cyst. The previous diagnosis of the follicular cyst was confirmed by ultrasonography and serum progesterone assay. Ultrasound images of the ovarian follicles, both mature and cystic follicles, were individually identified and monitored and were subsequently digitized for computer analysis of echotexture [mean pixel value (MPV) and pixel heterogeneity (PH)] of the follicle antrum and wall. Animals were classified into two groups (Treated, n= 20 and control, n= 11). The treated buffalo-cows injected im with 100mg progesterone oily in nature followed by im injection of 5000 IU HCG at days 5. Out of the twenty treated animals 14 buffaloes responded and diagnosed as they had luteinized cyst. Out of the 14 animals that had luteinized cyst 9 buffaloes injected with 25 mg of PGF2α at day 14 and the other 5 buffaloes left as control. The 6 non responded buffaloes were classified into two subgroups each (n= 3), one of them injected with HCG and the other left as control without any treatment. The time elapsed till luteinization of the follicular treated group was significantly different (32.67±1.45 days and 15.71±0.75 days respectively). The recovery rate was 27.27% in control animals and 70% in treated ones. Days elapsed till appearance of estrus was significantly shorter in treated buffaloes (22.20±1.19 days) in comparison to the control ones (56.33±2.03 days). The conception rates were 33.33% and 66.67% in control and treated group respectively.