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Glutathione peroxidase-1 and klotho gene expression in acute renal failure rats and its association with creatinine and urea levels.
Benha Veterinary Medical Journal • 2015
Publication Information
Authors
E Fatma, SH Hemeda, GA Sosa, F Naglaa
Keywords
Not Available
Journal
Benha Veterinary Medical Journal
Publisher
Not Available
Volume
29
Issue
Not Available
Pages
97-104
publication.type
Local
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
The main objectives of this work was to study the effect of glycerol induced acute renal injury on
Glutathione peroxidase (GPx-1) and klotho gene expression which used as early indicator for acute renal
failure. Twelve male albino rats divided into two groups were included in this study. Blood samples
were taken for assessment of creatinine and blood urea nitrogen. Kidney tissue specimens were
processed for mRNA gene expression study of Glutathione peroxidase (GPx-1) and klotho genes by real
time polymerase chain reaction (RT-PCR). The creatinine and blood urea nitrogen significantly
increased (4.97± 0.75 and 242.03 ± 41.93) compared with control group (0.67 ± 0 .06 and 24.43 ± 2.18).
Moreover, the renal Glutathione peroxidase-1 and klotho gene expression was found to be decreased in
the glycerol treated group (affected group), to 0.899 and 0.91fold. Also a correlation between gene
expression of Glutathione peroxidase-1and klotho gene with the level of creatinine and blood urea
nitrogen was detected.
Glutathione peroxidase (GPx-1) and klotho gene expression which used as early indicator for acute renal
failure. Twelve male albino rats divided into two groups were included in this study. Blood samples
were taken for assessment of creatinine and blood urea nitrogen. Kidney tissue specimens were
processed for mRNA gene expression study of Glutathione peroxidase (GPx-1) and klotho genes by real
time polymerase chain reaction (RT-PCR). The creatinine and blood urea nitrogen significantly
increased (4.97± 0.75 and 242.03 ± 41.93) compared with control group (0.67 ± 0 .06 and 24.43 ± 2.18).
Moreover, the renal Glutathione peroxidase-1 and klotho gene expression was found to be decreased in
the glycerol treated group (affected group), to 0.899 and 0.91fold. Also a correlation between gene
expression of Glutathione peroxidase-1and klotho gene with the level of creatinine and blood urea
nitrogen was detected.