Staff Members - Benha University
Effect of using ascorbic acid and cysteamine supplementation on in-vitro development of buffalo embryos
Asian Pacific Journal of Reproduction • 2017
Publication Information
Authors
Al-shimaa Al-HH El-Naby, Karima Gh M Mahmoud, Gamal AM Sosa, Mahmoud EA Abouel-Roos, Youssef F Ahmed
Keywords
Not Available
Journal
Asian Pacific Journal of Reproduction
Publisher
Not Available
Volume
6
Issue
Not Available
Pages
85-88
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Objective:
To improve in vitro embryo production in buffalo by supplementation of L-ascorbic acid during maturation and development (experiment 1) and combination with another antioxidant as cysteamine (experiment 2).
Methods:
Two experiments were performed, the first one aimed to evaluate the different concentrations (0, 25, 50, 100 μM) of L-ascorbic acid on embryo developmental rate of buffalo oocytes. The L-ascorbic acid was added to the maturation and culture media. In the second experiment, oocytes were cultured in media with two type of antioxidant (ascorbic acid+ cysteamine) or ascorbic acid only.
Results:
There was a significant increase in cleavage rate at 25, 50 μM than 100 μM and control group. But, the blastocyst rate was higher at 50 μM ascorbic acid than other concentrations (0, 25, 100 μM). Supplementation of ascorbic acid and cysteamine to maturation and cultured media improved
To improve in vitro embryo production in buffalo by supplementation of L-ascorbic acid during maturation and development (experiment 1) and combination with another antioxidant as cysteamine (experiment 2).
Methods:
Two experiments were performed, the first one aimed to evaluate the different concentrations (0, 25, 50, 100 μM) of L-ascorbic acid on embryo developmental rate of buffalo oocytes. The L-ascorbic acid was added to the maturation and culture media. In the second experiment, oocytes were cultured in media with two type of antioxidant (ascorbic acid+ cysteamine) or ascorbic acid only.
Results:
There was a significant increase in cleavage rate at 25, 50 μM than 100 μM and control group. But, the blastocyst rate was higher at 50 μM ascorbic acid than other concentrations (0, 25, 100 μM). Supplementation of ascorbic acid and cysteamine to maturation and cultured media improved