Cucumber (Cucumis sativus L.) is one of the most important economical crops, which belongs to family cucurbitaceae. The economic importance of this crop appears in both local consumption and exportation purposes. Cucumber is grown either in the open field or under protected agriculture houses. The purpose of growing crops under protected house conditions is to extend their cropping season and to protect them from adverse conditions as well as diseases and pests. Cucumber plants are affected by several fungal pathogens, and Fusarium oxysporum Schlechtend.:Fr. is among the most important pathogens. The causal agent of wilt disease in cucumber Fusarium oxysporum f.sp. cucumerinum is economically important wilting pathogen and causing significant yield losses in greenhouse of cucumber. The new trends now in the entire world in the field plant pathology aimed at developing alternative approaches for managing crop diseases to reducing use of fungicides in the control of diseases.