Background: Brucellosis is a major zoonoses affects wide range of domesticated as well as wild animals. Despite the
eradication program of brucellosis in Egypt, the disease is still endemic among cattle, buffaloes, sheep, goats, and
camels.
Results: In the present study, abortion occurred naturally among 25 animals (10 cows, 5 buffaloes, 9 Egyptian Baladi
goats and 1 ewe) shared the same pasture were investigated by real-time polymerase chain reaction (RT-PCR). DNA of
Brucella (B.) abortus was detected in serum of goats and sheep which has aborted recently by species-specific RT-PCR.
The results suggest cross-species infection of B. abortus from cattle to non-preferred hosts raised in close contact.
Conclusion: This article will renew our knowledge about the Brucella agent causing abortion in small ruminants in
Egypt. Information provided in this study is important for surveillance program, because eradication programs and
vaccination strategies may have to be adapted accordingly.