Background: Recently endometriosis is recognized as a challenging problem in gynecology and most common cause of infertility in young women representing 1 in 10 adolescents and women of reproductive age. Raising awareness of endometriosis in adolescence is receiving increasing attention now. Aim: The present research was aimed to evaluate the effect of self-instructional module on adolescent nursing students' awareness regarding endometriosis as challenge for prevention of future infertility. Design: A quasi-experimental research design was adopted to fulfill the aim of this study. Setting: The study was conducted at faculty of nursing, Benha University. Sample: A purposive sample of three hundred and sixty four adolescent female nursing students. Tools: Three main tools: A Structured self-administered questionnaire, Student's awareness assessment sheet regarding endometriosis and Student’s satisfaction sheet. Results: Showed that, less than one fifth of studied sample had adequate awareness score regarding endometriosis at pre-intervention phase while, about four fifths of the studied sample had adequate awareness score at post-intervention and at follow up phases (P ≤ 0.001). Also, there was no statistical significant relation between total awareness score of studied sample and all personal data (age, mothers' education, mothers' occupation, residence and family history of endometriosis) at post intervention and follow up phases p>0.05.Conclusion: The research concluded that, hypotheses of research were supported and adolescent female exhibited improving awareness about endometriosis after implementation of self-instructional module. Also, high satisfaction among adolescent students were achieved. Recommendations: Raising awareness regarding endometriosis through provision of the instructional module for all female university students.