Ten isolates of Salmonella species were obtained from sporadic cases of profuse diarrhea in new born ruminants from El-Menofiya and El-Kalubia Governorates in Egypt. These isolates were as follow 7 isolates from calves (Salmonella Typhimurium, Salmonella Enteritidis,two were Salmonella Saintpaul, two were Salmonella Langeveld and Salmonella Havana) ,two isolates from lambs (Salmonella Typhimurium, Salmonella Bardo) and one isolate from goat-kids(Salmonella Enteritidis). Salmonella isolates resistance to aminoglycosoides was 0.00% for Amikacin, 30% for tobramycin and 50% for streptomycin. Susceptibility of isolates to tetracycline was 50% for doxycycline. Results showed a high incidence of aminoglycosoides resistance gene aadB in 100% of the isolates while aadA2 genes in 40% of the isolates. Incidence of tetracycline resistance genes was 0.00% for tetA(B) and 80% for tetA(A). The difference between the results of this study and those from other regions in Egypt necessitate a complete survey overall the country to make a complete and clear map of salmonella servoars, their antibiotics susceptibility and in molecular characterization of resistance determinants in each region.