| publication name | Molecular detection of some virulence genes of S. aureus isolated from mastitic Cows by PCR. |
|---|---|
| Authors | Ashraf A. Abdel-Tawab; Ahmed A.A. Maarouf ; Fatma I. El-Hofy; Sameh A.H. Abbas |
| year | 2016 |
| keywords | Mastitis, S. aureus, PCR, virulence genes |
| journal | BVMJ |
| volume | 30 |
| issue | 1 |
| pages | 238-245 |
| publisher | Not Available |
| Local/International | International |
| Paper Link | Not Available |
| Full paper | download |
| Supplementary materials | Not Available |
Abstract
In this study a total of 1200 quarter milk samples were selected from 300 cows at different localities of El-Kaliobia Governorate were examined bacteriologically and revealed that 57 S.aureus isolated from both clinical and subclinical mastitis with incidence of 45.6% of total isolates. Molecular typing of S. aureus by PCR revealed that all isolates (100%) (12 random S.aureus isolates) were positive for the 16 SrRNA genes of S. aureus. Genotyping of virulence genes encoding factors of S. aureus revealed that two isolates were positive for (tst-1) gene (16.7%), three isolates positive for (etb) 25%, nine isolates positive for each of (icaD and femA) genes (75%), seven isolates positive for (hlg) (58.3%), eight isolates positive for (nuc) (66.7%).