Staff Members - Benha University
DETECTION OF VIRULENCE GENES IN AEROMONAS HYDROPHIL ISOLATED FROM RABBITS BY POLYMERASE CHAIN REACTION
BVMJ • 2014
Publication Information
Authors
1Ashraf, A. AbdEl-Tawab, 2Ahmed, A. A. Maarouf, 1Fatma, I. El-Hofy and
2Amany,O.S.Khalil
1Bacteriology, Immunology and Mycology Dep., Fac. Vet. Med. Benha Univ.2Animal Health Research
"Benha branch".
Keywords
AEROMONAS HYDROPHIL, RABBITS, PCR
Journal
BVMJ
Publisher
Faculty of Vet.Med. Benha Univ.
Volume
27
Issue
2
Pages
48-57
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
The present study was performed on 260 rabbits (48 diseased and 212 freshly dead ones) from rabbit
farms at Kaliobia Governorate for inspection of Aeromonas hydrophila. Samples were taken from these
rabbits (liver; heart blood; lung; intestine; kidney and spleen from each one) after clinical and
postmortem examinationfor bacteriological examination. The results revealed that, 195 A. hydrophila
strains (12.5%) were isolated, 13(0.8%) from48 diseased rabbits and 182(11.7%) from 212 freshly dead
ones. Moreover, higher rates of isolation of A. hydrophila from intestine (42.0%); liver (24.1%); heart
blood (14.9%); lung (9.2%); kidney (6.7%) and spleen (3.1%).Ciprofloxacin, Enrofloxacin, Gentamicin,
and Sulpha trimethoprim were the most proper antibiotics with the highest in vitro efficiency against
isolated A. hydrophila. PCR results showed that, aerolysin (aerA) toxin virulence gene was detected in
10 out of 14 studied strains (71.4%). While haemolysin (hly) toxin virulence gene was detected in 4 out
of 10 studied strains (40.0%).Moreover, the sequences obtained for aerolysin (aerA) gene had accession
number KM 592977atGenBankand were 94% to 98% identical to the corresponding GenBank
sequences. To the best of our knowledge, it may be the first record of studying the virulence genes of A.
hydrophila strains isolated from rabbits in Egypt.
farms at Kaliobia Governorate for inspection of Aeromonas hydrophila. Samples were taken from these
rabbits (liver; heart blood; lung; intestine; kidney and spleen from each one) after clinical and
postmortem examinationfor bacteriological examination. The results revealed that, 195 A. hydrophila
strains (12.5%) were isolated, 13(0.8%) from48 diseased rabbits and 182(11.7%) from 212 freshly dead
ones. Moreover, higher rates of isolation of A. hydrophila from intestine (42.0%); liver (24.1%); heart
blood (14.9%); lung (9.2%); kidney (6.7%) and spleen (3.1%).Ciprofloxacin, Enrofloxacin, Gentamicin,
and Sulpha trimethoprim were the most proper antibiotics with the highest in vitro efficiency against
isolated A. hydrophila. PCR results showed that, aerolysin (aerA) toxin virulence gene was detected in
10 out of 14 studied strains (71.4%). While haemolysin (hly) toxin virulence gene was detected in 4 out
of 10 studied strains (40.0%).Moreover, the sequences obtained for aerolysin (aerA) gene had accession
number KM 592977atGenBankand were 94% to 98% identical to the corresponding GenBank
sequences. To the best of our knowledge, it may be the first record of studying the virulence genes of A.
hydrophila strains isolated from rabbits in Egypt.