Staff Members - Benha University
Comparative evaluation of standard serological tests for diagnosis of ovine brucellosis
BVMJ • 2014
Publication Information
Authors
Ashraf, A. Abd El Tawwab 1, Fatma I. El Hofy1, Khoudair M.Ramadan.2, Fatma E. Harb.3
1. Dept. of Bacteriology, Mycology and Immunity. Fac. of Vet. Med., Benha Univ. 2. Dep. of Brucellosis.
Animal Health Research Institute (AHRI. 3. General Organization
Keywords
Brucella, serological tests, sheep and goats.
Journal
BVMJ
Publisher
Faculty of Vet.Med. Benha Univ.
Volume
27
Issue
2
Pages
423-429
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
One hundred blood samples (55 sheep and45 goats) (include 79 females and 21 males)were selected from
farms suffer from brucellosis and examined serologically by Buffered Acidified plate Antigen Test
(BAPAT), Rose Bengal plate Antigen test (RBPAT) , Tube Agglutination test (TAT), Complement Fixation
Test (CFT) and Immunochromatography assay (ICA). The results of BAPAT were (61.81%) and (73.33%),
RBPAT& TAT were (61.81%) and (66.66%) and CFT &ICA were (60%) and (66.66%) in sheep and goats
respectively for all tests. Moreover, the positive reactors in females among sheep and goats were (79.1%),
(76.56%), (81.25%), (80.95%) and (79.36%) using BAPAT, RBPAT, TAT, CFT and ICA respectively. The
sensitivity, specificity and agreement of BAPAT with CFT in sheep and goats were (92.06%, 75.67%, 86%)
respectively. The sensitivity, specificity and agreement of RBPT with CFT in sheep and goats were
(88.88%, 78.37%, 85%) respectively. The sensitivity, specificity and agreement of TAT with CFT in sheep
and goats were (84.12%, 70.27%, 79%) respectively. The sensitivity, specificity and agreement of ICA with
CFT in sheep and goats were (98.41%, 97.29%, 98%) respectively. The sensitivity, specificity of CFT was
100%. In conclusion the ICA proved to be the most accurate, cheapest, rapid and simplest test for diagnosis
of ovine brucellosis.
farms suffer from brucellosis and examined serologically by Buffered Acidified plate Antigen Test
(BAPAT), Rose Bengal plate Antigen test (RBPAT) , Tube Agglutination test (TAT), Complement Fixation
Test (CFT) and Immunochromatography assay (ICA). The results of BAPAT were (61.81%) and (73.33%),
RBPAT& TAT were (61.81%) and (66.66%) and CFT &ICA were (60%) and (66.66%) in sheep and goats
respectively for all tests. Moreover, the positive reactors in females among sheep and goats were (79.1%),
(76.56%), (81.25%), (80.95%) and (79.36%) using BAPAT, RBPAT, TAT, CFT and ICA respectively. The
sensitivity, specificity and agreement of BAPAT with CFT in sheep and goats were (92.06%, 75.67%, 86%)
respectively. The sensitivity, specificity and agreement of RBPT with CFT in sheep and goats were
(88.88%, 78.37%, 85%) respectively. The sensitivity, specificity and agreement of TAT with CFT in sheep
and goats were (84.12%, 70.27%, 79%) respectively. The sensitivity, specificity and agreement of ICA with
CFT in sheep and goats were (98.41%, 97.29%, 98%) respectively. The sensitivity, specificity of CFT was
100%. In conclusion the ICA proved to be the most accurate, cheapest, rapid and simplest test for diagnosis
of ovine brucellosis.