Staff Members - Benha University
Changes in Metabolite Profiling and Expression Levels of Key Genes Involved in the Terpenoid Biosynthesis Pathway in Garden Sage (Salvia officinalis) under the Effect of Hydrazine Hydrate
Metabolites • 2023
Publication Information
Authors
Mohammed Ali, Aisha M. Abdelkawy, Doaa Bahaa Eldin Darwish, Hanan Ali Alatawi, Dikhnah Alshehri, Hadba Al-Amrah and Fathia A. SoudyDikhnah Alshehri 3 , Hadba Al-Amrah 6 and Fathia A. Soudy 7
Keywords
Salvia officinalis; hydrazine hydrate (HZ); transcriptional control; chemical mutagens;
terpene synthase genes
Journal
Metabolites
Publisher
MDPI
Volume
13
Issue
(7)
Pages
807
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
Mutagenesis is a highly efficient tool for establishing genetic variation and is widely used
for genetic enhancement in various plants. The key benefit of mutation breeding is the prospect of
enhancing one or several characteristics of a variety without altering the genetic background. In this
study, we exposed the seeds of Salvia officinalis to four concentrations of hydrazine hydrate (HZ),
i.e., (0%, 0.1%, 0.2%, and 0.3%) for 6 h. The contents of terpenoid compounds in the S. officinalis
plantlets driven from the HZ-treated seeds were determined by GC-MS, which resulted in the identification
of a total of 340 phytochemical compounds; 163 (87.48%), 145 (84.49%), 65 (97.45%), and
62 (98.32%), from the four concentrations of HZ (0%, 0.1%, 0.2%, and 0.3%), respectively. Furthermore,
we used the qRT-PCR system to disclose the “transcriptional control” for twelve TPS genes
related to terpenoid and terpene biosynthesis, namely, SoGPS, SoMYRS, SoNEOD, SoCINS, SoSABS,
SoLINS, SoFPPS, SoHUMS, SoTPS6, SoSQUS, SoGGPS, and SoGA2. Altogether, results are likely to
ensure some positive relationship between the concentrations of the chemical mutagen HZ used
for treating the seeds, the type and amount of the produced terpenes, and the expression of their
corresponding genes.
for genetic enhancement in various plants. The key benefit of mutation breeding is the prospect of
enhancing one or several characteristics of a variety without altering the genetic background. In this
study, we exposed the seeds of Salvia officinalis to four concentrations of hydrazine hydrate (HZ),
i.e., (0%, 0.1%, 0.2%, and 0.3%) for 6 h. The contents of terpenoid compounds in the S. officinalis
plantlets driven from the HZ-treated seeds were determined by GC-MS, which resulted in the identification
of a total of 340 phytochemical compounds; 163 (87.48%), 145 (84.49%), 65 (97.45%), and
62 (98.32%), from the four concentrations of HZ (0%, 0.1%, 0.2%, and 0.3%), respectively. Furthermore,
we used the qRT-PCR system to disclose the “transcriptional control” for twelve TPS genes
related to terpenoid and terpene biosynthesis, namely, SoGPS, SoMYRS, SoNEOD, SoCINS, SoSABS,
SoLINS, SoFPPS, SoHUMS, SoTPS6, SoSQUS, SoGGPS, and SoGA2. Altogether, results are likely to
ensure some positive relationship between the concentrations of the chemical mutagen HZ used
for treating the seeds, the type and amount of the produced terpenes, and the expression of their
corresponding genes.