Staff Members - Benha University
HPLC Evaluation of Phenolic Profile, and Antioxidant Activity of Different Extracts of Jatropha curcas Leaves
Int. J. Pharm. Sci. Rev. Res. • 2014
Publication Information
Authors
Farouk K El-Baz, Frahat F Ali, Ahmed A Abd El-Rahman, Hanan F Aly3
, Safaa A Saad, Amal A Mohamed
Keywords
Antioxidant activity, DPPH, Flavonoid, J. curcas, Phenolic, Reducing power.
Journal
Int. J. Pharm. Sci. Rev. Res.
Publisher
Not Available
Volume
29
Issue
1
Pages
203-210
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
Petroleum ether, ethyl acetate, successive and crude methanolic extracts of Jatropha curcas leaves were evaluated for their
phytochemical screening, total polyphenol content and antioxidant activities. Qualitative phytochemical screening was tested and
showed positive results for flavonoids, tannins, phenolics, Saponins, Coumarins, sterols and triterpenes compounds. The highest total phenolic content (104.40±9.33 mg GAE/g d.w.) was found in successive methanolic extract. However, the highest total
flavonoid content (64.17±0.51 mg QUE/g d.w.) was detected in crude methanolic extract. The antioxidant activity was assessed by
using three assays; free radical scavenging activity (DPPH), total antioxidant activity and reducing power activity. The successive
methanolic extract exhibited the highest antioxidant activity in all assays. Individual polyphenolic compounds were evaluated in
successive methanolic extract using HPLC and the major compound detected was catechin (0.15%). Results confirmed the presence
of therapeutically potent compounds in the leaf extracts predominantly phenolic and flavonoid.
phytochemical screening, total polyphenol content and antioxidant activities. Qualitative phytochemical screening was tested and
showed positive results for flavonoids, tannins, phenolics, Saponins, Coumarins, sterols and triterpenes compounds. The highest total phenolic content (104.40±9.33 mg GAE/g d.w.) was found in successive methanolic extract. However, the highest total
flavonoid content (64.17±0.51 mg QUE/g d.w.) was detected in crude methanolic extract. The antioxidant activity was assessed by
using three assays; free radical scavenging activity (DPPH), total antioxidant activity and reducing power activity. The successive
methanolic extract exhibited the highest antioxidant activity in all assays. Individual polyphenolic compounds were evaluated in
successive methanolic extract using HPLC and the major compound detected was catechin (0.15%). Results confirmed the presence
of therapeutically potent compounds in the leaf extracts predominantly phenolic and flavonoid.