PRODUCTION AND EVALUATION OF CELLULASE ENZYMES FROM
TRICHODERMA HAMA TUM AND RHIZOPUS NIGMCANS
BY
Foda, F.F. and Abd EleAleem, L M.
Dept. of Agric. Biochein., Fac. of Agric., Moshiohor, Zagazig Univer. Banha
Branch
ABSTRACT
a
Two different fungi strains were used for production of cellulase
enzymes. Trichoderma Hamalum was found to be the best cellulase producer.
The optimum conditions for cellulase production using two different fungi was
found to be by mandel ‘ s media (No. 1) with (I % w/v) cellulose powder and (1 %
w/v) carboxymethyl cellulose as carbon source, at pH 58 and incubation for 9
days at 3O’ C. The final activity of carboxy methyl cellulase (CMC ase) and filter
paper activity (FP ase) were I 3 . 1 U/nil and 1 b7 U/ml for Trichoderma Harnatum
4 6.4 UI ml and I. 1 UI ml for Rhizopus nigricans by using CMC in Mandel’s
media after 9 days. However. the CMCase and FPase were 14.3 U/ml and 3.3
U/mal for Trichoderma Hamatum, 8.9 U/ml and 2.7 U/ml for Rhizopus nigri cans
by using cellulose powder in Mandel’s media at the same incubation time. The
relaْve activity of cellulase enzymes were 93 .8 % and 9 1 .4 % for Tri choderma
Hamalum and Rhizopus nigricans , respectively after 63 days incubation time at
40 • The optimum pH values were 5.0 for the first and 5.5 for the second and
the optimum temperature was 55° C and 500 C for two fungi. respectively. The
optimum enzyme concentrations for both were 0.5 ml (0.07-O. I mg proteinlmL)
cultural filtrate. The Iا values were 2 mg/ml and 0.18 mg/rn] and maximum
reaction velocity (V) were 163.7 mMJL and 1 15.3 mM/L for Tri choderma
Hamatum and Rhizopus nigricans, respectively.