This investigation was conducted to study the pattern of brucella agglutination tietre (BAT) among workers in some occupations with possible exposure to infected animals, and to study some factors that might be related to the development of brucellosis. It was found that 9.94% of the exposed workers (no. = 483) had BAT ≥ 1/200, and 10.14% had BAT ≥ 1/l00. Veterinarians, butchers and abattoir workers had the highest prevalence of (BAT) (≥ 1/100 & 1/200). Age of the workers had no relation with the level of BAT. A difference between males and females was detected concerning the agglutination titre, but it was not a true one. Male workers were involved in violent jobs (Slaughtering and Skinning) which were characterized by high titres, these jobs could not be tolerated by the females. The percentage of rural residents with high (BAT) was higher than among urban residents (23.26% and 15.38% respectively). Duration of exposure to the infected animals had no relation with (BAT) or with the development of manifestations of brucellosis.