Staff Members - Benha University
Molecular identification of M. bovis BCG by Multiplex PCR
Benha veterinary medicine J • 2016
Publication Information
Authors
Ashraf A. Abd El-Tawab, Fatma I. El-Hofy, Essam A. Nasr, Nammalwar Sriranganathan and Enas A. Soliman.
Keywords
Region of difference, BCG, PCR.
Journal
Benha veterinary medicine J
Publisher
Not Available
Volume
31
Issue
1
Pages
119-123
publication.type
Local
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
Mycobacterium bovis (M. bovis) BCG belongs to Mycobacterium tuberculosis complex (MTBC), highly related
organisms, which are 99.9 % similar at nucleotide level and phenotypically similar. Its differentiation from other members
of MTBC by conventional methods is laborious and time consuming. PCR provides a rapid alternative method for
differentiation between members of MTBC. It depends on identification of region of difference (RD) which have been
lost during attenuation of M. bovis. Two different BCG strains (from two sources) were confirmed as a member of M.
tuberculosis (MTB) complex (MTC) and as BCG strains by PCR using primers to a region of the 16S rRNA gene that is
conserved in all mycobacteria and region of difference (RD1, RD4, RD9 and RD12) respectively. DNA of Mycobacterium
tuberculosis was used as a control to compare with BCG strains. The results showed that 16S rRNA gene was present in
all tested strains, while the RD1, RD4, RD9 and RD12 were absent only in BCG strains.
organisms, which are 99.9 % similar at nucleotide level and phenotypically similar. Its differentiation from other members
of MTBC by conventional methods is laborious and time consuming. PCR provides a rapid alternative method for
differentiation between members of MTBC. It depends on identification of region of difference (RD) which have been
lost during attenuation of M. bovis. Two different BCG strains (from two sources) were confirmed as a member of M.
tuberculosis (MTB) complex (MTC) and as BCG strains by PCR using primers to a region of the 16S rRNA gene that is
conserved in all mycobacteria and region of difference (RD1, RD4, RD9 and RD12) respectively. DNA of Mycobacterium
tuberculosis was used as a control to compare with BCG strains. The results showed that 16S rRNA gene was present in
all tested strains, while the RD1, RD4, RD9 and RD12 were absent only in BCG strains.