Blood culture is the most important tool for detecting bacteremia in children with fever. However, contaminated blood cultures have been recognized as a troublesome issue for decades and continue to be a source of frustration for clinical and laboratory personnel alike. The purpose of this study was to reduce blood culture contamination rates after conducting intervention measures during blood culture collection in patients aged 14 years who visited AL-Quwayiyah General Hospital, Riadh, KSA. Rate of contamination of blood culture and the causative bacteria data was obtained from microbiology register in the period from January 2014 to June 2014 (Pre-intervention phase), there were 94 positive cultures. Of these, 33 (35 %) grew contaminant organisms. The proportion of all blood cultures obtained during this period that grew contaminant organisms was 33 (4.4%) of 754. After conducting the intervention measures during the period from August 2014 to January 2015 (post-intervention phase), there were 49 positive cultures. Of these, 12 (24.5%) grew contaminant organisms. The proportion of all blood cultures that grew contaminant organisms decreased to 12 (1.7%) of 691 compared to Preintervention phase contaminated cultures 32 (4.3%) (p < 0.001). Overall, contamination rates were higher in younger children than in older children, given the difficulty of performing blood sampling in younger children