Background: Urinary tract infection (UTI) is the most frequent and serious issue with urinary catheters. Aim: was to evaluate the effect of implementing educational programs on reducing urinary tract infections among patients with urinary catheterization. Methods: Quasi-experimental design was used. Setting: the study was conducted at the urology department at Benha University Hospitals. subjects: all available nurses (30) working in the urology department and a purposive sample of 60 adult patients in need of urethral catheterization for more than seven days, Equally divided randomly into (30) a study group and (30) control group. Three tools were used for data collection including nurses’ knowledge assessment questionnaire, nurses' practice observational checklist regarding the care of long-term urinary catheters, and patients’ social and biomedical data. The results: The control group's positive urine culture rate (86%) was greater than the study group's rate (6.6 percent ). The outcomes also showed that nurses' understanding and practice of catheter patient care considerably increased following the implementation of the nursing education program. Conclusion: The implementation of the nursing training program reduces the incidence of urinary tract infections in catheterized patients, and there are notable variations in the knowledge and performance of nurses before and after its implementation. The study recommended that to avoid catheter-related urinary tract infections, establish treatment guidelines. A written training program on catheter use, insertion, removal, and maintenance should be provided and put into practice. Ensure you have the tools needed for sterile catheterization on hand. Make sure there are enough skilled nurses and technical resources available to reduce catheter-related UTI occurrence and promote outcomes.