Women after menopause have a lot of complains that negatively distress their life. In the modern years, a lot of conduction
methods have been presented to relief undesirable symptoms. Bone marrow mesenchymal stem cells (BM-MSCs) can be
recently used as a new therapeutic method in treatment of many diseases and avoidance the hormonal sides effects that
happened after menopause.
Aim: The aim of this research is to show a new approach in modification of the structure of vaginal mucosal atrophy by using
the (BM-MSCs) in induced ovariectomized rats.
Methods and Results: Fifty-five female albino rats were used and divided randomly into five groups: control group,
ovariectomized group, ovariectomized group plus estrogen (20ug /kg/day for 4 weeks), ovariectomized rats with BM-MSCs
group (5 × 106 MScs/rat intravenously) and ovariectomized rats with stem cells /BM-MSCs Intra-vaginal group, the expression
of genes for GAPDH, iNOS and TGF-β were done and vaginal biopsies were taken for histological and immunohistochemical
studies. In ovariectomized group there was inflammation, ulceration with irregularity in collagen fibers, decreased estrogen
receptors expression and the expressions of TGF-β, GAPDH and iNOS were very high. While the rate of healing of epithelium
was increased with increased in the vasculatures of vaginal mucosa and the estrogen receptors expression was high with
decreased expression of GAPDH, iNOS and TGF-β in ovariectomized rats that treated with intra- vaginal BM-MSCs.
Conclusions: Using the BM-MSCs could be used intravaginal safely in case of vaginal atrophy as they modify the structure of
vaginal mucosa superior to estrogen hormone therapy.