This cross sectional short term study was carried out on 120 primiparous mothers attending Benha maternal & child health center to assess their knowledge and practices towards their infants’ care.The results of this study showed that the majority of the studied group had inadequate knowledge as well as poor practices regarding items used for assessment. Urban mothers showed higher mean percent of correct answers regarding knowledge about proper child health care. They also illustrated higher mean percent of correct practices for diaper care as well as for proper breast feeding practices. Educated primiparous mothers showed higher mean percentage of correct answers regarding child health, accident protection as well as knowledge about infants’ immunization. Working mothers showed higher mean percent of correct practices regarding frequency of diaper change as well as baby bath. So, it is of great importance to improve the quality of infant care provided via maternal and child health centers through health system reforming.