Background: Moringa oleifera is high valued plant and used in many countries around the world. The seed of Moringa oleifera (MO) is an important part and has a remarkable medicinal, nutritional and socio-economic values, this study, therefore, was designed to clarify the protective effect of Moringa oleifera hydroethanolic seed extract (MOSE) against carbon tetrachloride (CCl4) induced hepatoxicity and hemotoxicity in rats. Methods: A total of one hundred and five male rats were randomly divided into 7 groups of 15 rats each. The hydroethanolic seed extract (30%) was administered orally for one month at 250 and 500mg/kg body weight. Samples were collected after day1,15 and 30 post administration. Results: Phytochemical, biochemical, hematological and hisopathological examinations were utilized to investigate hepatoprotective activity of MOSE. The results obtained demonstrated that, phytochemicals such as alkaloids, glycosides, anthraquinones, tannins, flavonoids, gum, resin, saponins, terponoids, protein and fats were detected in the seeds. Treatment with the MOSE caused a significant (P