A total of 27 adult Ossimi ewes were divided into three groups (9 ewes/ each). The 1st group, which served as a control, was treated with distilled water (10 mL/kg B.W. /day), the 2nd group was treated with selenium nanoparticles (SeNPs 1 mg/kg B.W./day), and the 3rd group was treated with L-Carnitine (L-Car 350 mg/Kg/day). Data revealed that triglyceride levels decreased at 45 days of postpartum due to SeNPs treatment, while total cholesterol increased at 11 days and decreased at 45 days of postpartum due to SeNPs and L-Car. HDL levels increased at 11 days and decreased at 60 days postpartum by using SeNPs and L-Car. LDL decreased at day 11 by L-Car administration. AST decreased at 11, 45, and 60 days in the SeNPs group and ALT decreased at day 11 in the treated group and decreased at day 45 in the L-Car group. Urea levels decreased at day 11 in treated groups. On the other hand, creatinine levels decreased at days 45 and 60 of postpartum in treated groups. SeNPs increase SOD along postpartum and decreased GSSG at days 11 and 60 and increased at day 45, while, L-Car decreased GSSG at days 11 and 60 and increased at day 45 of postpartum. NO concentrations decreased along postpartum in treated groups and increased in ATP at mid and late postpartum in treated groups. As a result, SeNPs and L-Car oral administration enhanced various biochemical parameters and serum antioxidant activities for Ossimi ewes during the post-partum periods.